Title | Determination of a complete set of coupling constants in 13C-labeled oligonucleotides. |
Publication Type | Journal Article |
Year of Publication | 1994 |
Authors | Schwalbe H, Marino JP, King GC, Wechselberger R, Bermel W, Griesinger C |
Journal | J Biomol NMR |
Volume | 4 |
Pagination | 631–644 |
Date Published | Sep |
Accession Number | 9 |
Abstract | Three experiments are introduced to determine a complete set of coupling constants in RNA oligomers. In the HCCH-E.COSY experiment, the vicinal proton-proton coupling constants can be measured with high accuracy. In the P-FIDS-CT-HSQC experiment, vicinal proton-phosphorus and carbon-phosphorus couplings are measured that depend on the phosphodiester backbone torsion angles beta and epsilon. In the refocussed HMBC experiment, vicinal carbon-proton couplings are measured that depend on the glycosidic torsion angle chi. |