Isotope labeling of mammalian GPCRs in HEK293 cells and characterization of the C-terminus of bovine rhodopsin by high resolution liquid NMR spectroscopy.

TitleIsotope labeling of mammalian GPCRs in HEK293 cells and characterization of the C-terminus of bovine rhodopsin by high resolution liquid NMR spectroscopy.
Publication TypeJournal Article
Year of Publication2008
AuthorsWerner K, Richter C, Klein-Seetharaman J, Schwalbe H
JournalJ Biomol NMR
Volume40
Pagination49–53
Date PublishedJan
Accession Number149
Abstract

High amino acid coverage labeling of the mammalian G protein coupled receptors (GPCR) rhodopsin was established with 15N and 15N/13C isotopes. Rhodopsin was expressed at preparative scale in HEK293S cells and studied in full-length by NMR spectroscopy in detergent micelle solution. This resulted in the assignment and detailed study of the dynamic properties of the C-terminus of rhodopsin. The rhodopsin C-terminus is immobilized until Ala333, after which it becomes unstructured.
URLhttp://dx.doi.org/10.1007/s10858-007-9205-3
DOI10.1007/s10858-007-9205-3