Title | Determination of HN,Hα and HN,C′ coupling constants in 13C, 15N-labeled proteins |
Publication Type | Journal Article |
Year of Publication | 1994 |
Authors | Weisemann R, Rüterjans H, Schwalbe H, Schleucher J, Bermel W, Griesinger C |
Journal | Journal of Biomolecular NMR |
Volume | 4 |
Issue | 2 |
Pagination | 231 - 240 |
Date Published | 1994/03/01/ |
Accession Number | 8 |
Abstract | Sensitive three-dimensional NMR experiments, based on the E.COSY principle, are presented for the measurement of the 3J(HN,Ha) and 3J(HN,C') coupling constants in uniformly 13C- and 15N-labeled proteins. They employ gradient coherence selection in combination with the sensitivity enhancement method in HSQC-type spectra (Cavanagh et al., 1991; Palmer et al., 1991). In most cases, the two measured coupling constants unambiguously define the ?-angle for protein structure determination. The method is applied to uniformly 13C, 15N-labeled ribonuclease T1. |
URL | http://dx.doi.org/10.1007/BF00175250 |