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Determination of HN,Hα and HN,C′ coupling constants in 13C, 15N-labeled proteins

TitleDetermination of HN,Hα and HN,C′ coupling constants in 13C, 15N-labeled proteins
Publication TypeJournal Article
Year of Publication1994
AuthorsWeisemann R, Rüterjans H, Schwalbe H, Schleucher J, Bermel W, Griesinger C
JournalJournal of Biomolecular NMR
Volume4
Issue2
Pagination231 - 240
Date Published1994/03/01/
Accession Number8
Abstract

Sensitive three-dimensional NMR experiments, based on the E.COSY principle, are presented for the measurement of the 3J(HN,Ha) and 3J(HN,C') coupling constants in uniformly 13C- and 15N-labeled proteins. They employ gradient coherence selection in combination with the sensitivity enhancement method in HSQC-type spectra (Cavanagh et al., 1991; Palmer et al., 1991). In most cases, the two measured coupling constants unambiguously define the ?-angle for protein structure determination. The method is applied to uniformly 13C, 15N-labeled ribonuclease T1.

URLhttp://dx.doi.org/10.1007/BF00175250