Chemical synthesis of 13C-labelled monomers for the solid-phase and template controlled enzymatic synthesis of DNA and RNA oligomers
Title | Chemical synthesis of 13C-labelled monomers for the solid-phase and template controlled enzymatic synthesis of DNA and RNA oligomers |
Publication Type | Journal Article |
Year of Publication | 1994 |
Authors | Quant S, Wechselberger RW, Wolter MA, W, Schell P, Engels JW, Griesinger C, Schwalbe H |
Journal | Tetrahedron Lett |
Volume | 35 |
Issue | 36 |
Pagination | 6649 - 6651 |
Date Published | 1994/9/5/ |
Accession Number | 12 |
Abstract | The preparation of 13C-labelled ribonucleosides starting from [13C6]-glucose 1 and the corresponding nucleobases 5a-e or 6a-e (N6-benzoyl-adenine, N2-acetyl-guanine, N4-benzoyl-cytosine, uracil and thymine) in 47 - 66% overall yield is described. Their subsequent transformation into 5'-O-dimethoxytrityl protected DNA-phosphoramidites and 5'-O-dimethoxytrityl-2'-O-trialkylsilyl protected RNA-phosphor-amidites for the solid phase synthesis of DNA- and RNA-oligomers and to 5'-O-ribo- and deoxyribo-nucleosidetriphosphates for template controlled enzymatic synthesis (polymerase- or reverse transcriptase reaction) has been carried out. |
URL | http://www.sciencedirect.com/science/article/B6THS-42HXDJW-16C/2/d5e89170fb40aa606a9cc73dbe83569c |